The COY® O2 Control Glove Box for cell growth and imaging streamlines life science laboratory operations by enabling researchers to harvest, culture, isolate, maintain and image cells while controlling temperature, humidity, CO2 and O2. The glove box design eliminates experimental error due to environmental disruption because workspace and microscope are in the same environment and cultures can go directly from incubation to the microscope. The glove box contains both a workspace for cell handling and a separate cabinet that functions as a humidified incubation box. The user's microscope sits outside the incubation box yet inside the glove box in a non-condensing environment. Oculars are readily accessible through a microscope viewport that fits into the wall of the glove box. Easy manipulation of microscope controls can be accomplished with direct contact through a unique sleeve access system that allows barehanded sample manipulation. Control ranges include temperatures of plus 4 ambient degrees C to 40°C, O2 levels of 0 percent to 60 percent with 0.1 percent resolution, and CO2 levels of 0 percent to 20 percent with 0.1 percent resolution. The glove box dehumidifier removes excess moisture from the system to create a non-condensing environment. The humidified incubation box within the glove box is saturated with the same temperature, O2 and CO2 present in the rest of the glove box.