Supplied as silicone fittings attached directly to silicone tubing or hose, the assemblies are well-suited for applications involving aseptic transfer, sterile processing, and other critical applications in the pharmaceutical, biomedical, and high purity industries. The assemblies eliminate entrapment issues and leaks associated with barb-type fittings; better meet requirements for process validations and inspections; provide a smooth inner surface with a seamless transition; offer consistent interior dimensions for maximum flow rates; reduce end-user assembly times, assemblies are ready to install; no contact with metallic materials. Platinum-cured silicone features temperature resistance from -100°F to 400°F. The assemblies do not impart taste or odor, nor do they contain plasticizers, which can leach out and cause contamination.